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Biochem Biophys Res Commun. 2016 Oct 14;479(2):224-230. doi: 10.1016/j.bbrc.2016.09.046. Epub 2016 Sep 13.

PPARγ is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation.

Author information

1
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China.
2
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, PR China; Cunji Medical School, University of Chinese Academy of Sciences, PR China.
3
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, PR China; College of Life Science, Hebei University, 071000, PR China.
4
Rongchang Breeding Company, Shandong, Binzhou, 251900, PR China.
5
Tsinghua University High School, Beijing, 100094, PR China.
6
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China. Electronic address: wangyanfang@caas.cn.

Abstract

To elucidate the key miRNAs and the signalling pathways that are involved in porcine oocyte maturation, we performed a deep sequencing analysis of the miRNAs of pig germinal vesicle (GV) oocytes and metaphase II (MII) oocytes. Seven differentially expressed (DE) miRNAs were identified and the expression levels of miR-21 and miR-27b-3p were further confirmed by QPCR analysis. The target genes of 7 DE miRNAs were predicted and subjected to pathway analysis. Interestingly, fatty acid metabolism and fatty acid biosynthesis were the top two significantly enriched molecular functions during oocyte maturation. Heat map, which was built with 7 DE miRNAs and the enriched the molecular functions, revealed that miR-21, miR-27b-3p, miR-10a-5p and miR-10b-5p were involved in fatty acid metabolism. In particular, the regulatory role of miR-27b-3p on peroxisome proliferator-activated receptor-γ (PPARγ) was confirmed by their inversed expression patterns in GV and MII oocytes and luciferase report assays. In addition, we observed that PPARγ agonist (rosiglitazone) treatment significantly enhanced porcine oocyte maturation rate and early embryo developmental competent. Taken together, our results demonstrated that miR-27b and its target, PPARγ, play the vital roles in pig oocyte maturation through regulating the fatty acid metabolism. These data increased our understanding of the regulatory gene networks in porcine oocyte maturation and development.

KEYWORDS:

Fatty acid metabolism; Oocyte maturation; PPARγ; Pig; miRNA

PMID:
27638309
DOI:
10.1016/j.bbrc.2016.09.046
[Indexed for MEDLINE]

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