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Comp Immunol Microbiol Infect Dis. 2016 Oct;48:41-7. doi: 10.1016/j.cimid.2016.07.006. Epub 2016 Jul 26.

Clinical improvement in feline herpesvirus 1 infected cats by oral low dose of interleukin-12 plus interferon-gamma.

Author information

1
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy; Department of Chemistry, Istituto Zooprofilattico del Mezzogiorno, Portici, Naples, Italy. Electronic address: filomena.fiorito@unina.it.
2
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
3
Veterinary Clinic Noè, Telese Terme, Benevento, Italy.
4
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy. Electronic address: luisa.demartino@unina.it.
5
CNR, Institute of Cybernetics, Pozzuoli, Naples, Italy.
6
Clinical Research Unit, GUNA S.p.a., Milan, Italy.

Abstract

Feline herpesvirus 1 (FHV-1) is a widespread cat pathogen inducing rhinitis, conjunctivitis and corneal ulcers. To alleviate acute FHV-1-induced disease, antiviral agents are used often with antibiotics. But sometimes, these treatments, as well as conventional doses of cytokines have moderate efficacy and/or collateral effects. Herein we have investigated the effects of low dose interleukin (IL)-12 plus interferon (IFN)-gamma, prepared by Sequential Kinetic Activated (SKA), on the treatment of FHV-1 infection. Twenty-five, unvaccinated FHV-1-positive cats were recruited into a prospective, randomized, placebo-controlled, double-blinded clinical trial. Fifteen cats were treated for 6 months with oral low doses of SKA IL-12 plus IFN-gamma and 10 cats were treated with placebo. At 1, 6 and 12 months (follow-up) after the beginning of treatment, clinical assessment, PCR assay and blood count were carried out. At follow-up, in treated group, we observed significant (p<0.05) improvements in clinical signs and PCR became negative in 12/15 cats (80%). In placebo, 10/10 cats were PCR-positive, with improvements (30%) or worsening (70%) in clinical signs. Blood values were normal in both groups. Our results show that the low dose therapy, based on activated solutions of IL-12 plus IFN-gamma, represents a novel approach to treat FHV-1 infection in cats.

KEYWORDS:

Cats; Clinical trial; Feline herpesvirus-1; Interleukin-12 plus interferon-gamma; Low dose therapy; Sequential Kinetic ActivationSequential kinetic activation

PMID:
27638118
DOI:
10.1016/j.cimid.2016.07.006
[Indexed for MEDLINE]

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