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Eur J Pharm Biopharm. 2016 Nov;108:187-195. doi: 10.1016/j.ejpb.2016.09.008. Epub 2016 Sep 12.

Enhanced acute anti-inflammatory effects of CORM-2-loaded nanoparticles via sustained carbon monoxide delivery.

Author information

1
College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea.
2
Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, England, United Kingdom.
3
Department of Neurosurgery, CHA University, CHA Bundang Medical Center, Seongnam, Gyeonggi 13496, Republic of Korea.
4
College of Pharmacy, Ajou University, Suwon, Gyeonggi 16499, Republic of Korea.
5
College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea. Electronic address: onbae@hanyang.ac.kr.
6
College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea. Electronic address: jinkikim@hanyang.ac.kr.

Abstract

The aim of this study was to enhance the anti-inflammatory effects of carbon monoxide (CO) via sustained release of CO from carbon monoxide-releasing molecule-2-loaded lipid nanoparticles (CORM-2-NPs). CORM-2-NPs were prepared by hot high pressure homogenization method using trilaurin as a solid lipid core and Tween 20/Span 20/Myrj S40 as surfactant mixture. The physicochemical properties of CORM-2-NPs were characterized and CO release from CORM-2-NPs was assessed by myoglobin assay. In vitro anti-inflammatory effects were evaluated by nitric oxide assay in lipopolysaccharide-stimulated RAW 264.7 macrophages. In vivo anti-inflammatory activity was investigated by measuring paw volumes and histological examination in carrageenan-induced rat paw edema. Spherical CORM-2-NPs were around 100nm with narrow particle size distribution. The sustained CO release from CORM-2-NPs was observed and the half-life of CO release increased up to 10 times compared with CORM-2 solution. CORM-2-NPs showed enhanced in vitro anti-inflammatory effects by inhibition of nitric oxide production. Edema volume in rat paw was significantly reduced after treatment with CORM-2-NPs. Taken together, CORM-2-NPs have a great potential for CO therapeutics against inflammation via sustained release of CO.

KEYWORDS:

Anti-inflammatory effect; CORM-2; Carbon monoxide; Lipid nanoparticles; Sodium dithionite (PubChem CID: 24489); Sustained release; Tricarbonyldichlororuthenium(II) dimer (PubChem CID: 10951331); Trilaurin (PubChem CID: 10851); Tween 20 (PubChem CID: 443314)

PMID:
27634645
DOI:
10.1016/j.ejpb.2016.09.008
[Indexed for MEDLINE]

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