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Asian Pac J Trop Med. 2016 Sep;9(9):844-850. doi: 10.1016/j.apjtm.2016.07.004. Epub 2016 Jul 26.

Prediction of promiscuous T-cell epitopes in the Zika virus polyprotein: An in silico approach.

Author information

1
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology, Islamabad 44000, Pakistan.
2
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology, Islamabad 44000, Pakistan. Electronic address: javedaneela@gmail.com.
3
Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan.
4
Foundation University Medical College, Foundation University Islamabad, DHA-I, Islamabad 44000, Pakistan. Electronic address: yasir_waheed_199@hotmail.com.

Abstract

OBJECTIVE:

To predict immunogenic promiscuous T cell epitopes from the polyprotein of the Zika virus using a range of bioinformatics tools. To date, no epitope data are available for the Zika virus in the IEDB database.

METHODS:

We retrieved nearly 54 full length polyprotein sequences of the Zika virus from the NCBI database belonging to different outbreaks. A consensus sequence was then used to predict the promiscuous T cell epitopes that bind MHC 1 and MHC II alleles using PorPred1 and ProPred immunoinformatic algorithms respectively. The antigenicity predicted score was also calculated for each predicted epitope using the VaxiJen 2.0 tool.

RESULTS:

By using ProPred1, 23 antigenic epitopes for HLA class I and 48 antigenic epitopes for HLA class II were predicted from the consensus polyprotein sequence of Zika virus. The greatest number of MHC class I binding epitopes were projected within the NS5 (21%), followed by Envelope (17%). For MHC class II, greatest number of predicted epitopes were in NS5 (19%) followed by the Envelope, NS1 and NS2 (17% each). A variety of epitopes with good binding affinity, promiscuity and antigenicity were predicted for both the HLA classes.

CONCLUSION:

The predicted conserved promiscuous T-cell epitopes examined in this study were reported for the first time and will contribute to the imminent design of Zika virus vaccine candidates, which will be able to induce a broad range of immune responses in a heterogeneous HLA population. However, our results can be verified and employed in future efficacious vaccine formulations only after successful experimental studies.

KEYWORDS:

Antigenicity; B-cell epitopes; T-cell epitopes; Vaccine; Zika virus

PMID:
27633296
DOI:
10.1016/j.apjtm.2016.07.004
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