Format

Send to

Choose Destination
Genes Dev. 2016 Sep 1;30(17):2005-17. doi: 10.1101/gad.287094.116. Epub 2016 Sep 15.

Temperature regulates splicing efficiency of the cold-inducible RNA-binding protein gene Cirbp.

Author information

1
Department of Molecular Biology, University of Geneva, CH-1211 Geneva 4, Switzerland;
2
The Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Swiss Institute of Bioinformatics, CH-1015 Lausanne, Switzerland.

Abstract

In mammals, body temperature fluctuates diurnally around a mean value of 36°C-37°C. Despite the small differences between minimal and maximal values, body temperature rhythms can drive robust cycles in gene expression in cultured cells and, likely, animals. Here we studied the mechanisms responsible for the temperature-dependent expression of cold-inducible RNA-binding protein (CIRBP). In NIH3T3 fibroblasts exposed to simulated mouse body temperature cycles, Cirbp mRNA oscillates about threefold in abundance, as it does in mouse livers. This daily mRNA accumulation cycle is directly controlled by temperature oscillations and does not depend on the cells' circadian clocks. Here we show that the temperature-dependent accumulation of Cirbp mRNA is controlled primarily by the regulation of splicing efficiency, defined as the fraction of Cirbp pre-mRNA processed into mature mRNA. As revealed by genome-wide "approach to steady-state" kinetics, this post-transcriptional mechanism is widespread in the temperature-dependent control of gene expression.

KEYWORDS:

Cirbp; circadian rhythms; splicing efficiency; temperature

PMID:
27633015
PMCID:
PMC5066242
DOI:
10.1101/gad.287094.116
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center