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Zebrafish. 2017 Feb;14(1):69-72. doi: 10.1089/zeb.2016.1321. Epub 2016 Sep 15.

A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease.

Author information

1
1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
2
2 Institute for Molecular Bioscience, University of Queensland , Brisbane, Australia .
3
3 ANZAC Research Institute, Concord Hospital, University of Sydney , Sydney, Australia .

Abstract

Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.

KEYWORDS:

Tol2 gateway; disease model; neurodegenerative disease; transgenesis

PMID:
27631880
DOI:
10.1089/zeb.2016.1321
[Indexed for MEDLINE]

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