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Molecules. 2016 Sep 12;21(9). pii: E1223. doi: 10.3390/molecules21091223.

Synergistic Antipseudomonal Effects of Synthetic Peptide AMP38 and Carbapenems.

Author information

1
Molecular Microbiology and Antibiotics, Department of Pathology and Experimental Therapeutics, Medical School-IDIBELL, University of Barcelona, Hospitalet, Barcelona 08907, Spain. hectorrudilla@gmail.com.
2
Molecular Microbiology and Antibiotics, Department of Pathology and Experimental Therapeutics, Medical School-IDIBELL, University of Barcelona, Hospitalet, Barcelona 08907, Spain. esterfustedominguez@ub.edu.
3
Department of Public Health, Mental Health and Perinatal Nursing, US of Nursing, University of Barcelona, Hospitalet, Barcelona 08907, Spain. esterfustedominguez@ub.edu.
4
Department of Physical Chemistry and Institute of Nanoscience and Nanotechnology, University of Barcelona, Barcelona 08028, Spain. ycajal@ub.edu.
5
Department of Organic Chemistry, University of Barcelona, Barcelona 08028, Spain. frabanal@ub.edu.
6
Molecular Microbiology and Antibiotics, Department of Pathology and Experimental Therapeutics, Medical School-IDIBELL, University of Barcelona, Hospitalet, Barcelona 08907, Spain. tvinuesa@ub.edu.
7
Molecular Microbiology and Antibiotics, Department of Pathology and Experimental Therapeutics, Medical School-IDIBELL, University of Barcelona, Hospitalet, Barcelona 08907, Spain. mvinyas@ub.edu.
8
Cooperativa De Ensino Superior Politécnico Universitário, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde (CESPU, IINFACTS), Gandra 4585-116, Portugal. mvinyas@ub.edu.

Abstract

The aim was to explore the antimicrobial activity of a synthetic peptide (AMP38) and its synergy with imipenem against imipenem-resistant Pseudomonas aeruginosa. The main mechanism of imipenem resistance is the loss or alteration of protein OprD. Time-kill and minimal biofilm eradication concentration (MBEC) determinations were carried out by using clinical imipenem-resistant strains. AMP38 was markedly synergistic with imipenem when determined in imipenem-resistant P. aeruginosa. MBEC obtained for the combination of AMP38 and imipenem was of 62.5 μg/mL, whereas the MBEC of each antimicrobial separately was 500 μg/mL. AMP38 should be regarded as a promising antimicrobial to fight MDR P. aeruginosa infections. Moreover, killing effect and antibiofilm activity of AMP38 plus imipenem was much higher than that of colistin plus imipenem.

KEYWORDS:

Pseudomonas; antimicrobial peptides; biofilm eradication; synergism

PMID:
27626405
DOI:
10.3390/molecules21091223
[Indexed for MEDLINE]
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