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Cell Prolif. 2016 Dec;49(6):720-728. doi: 10.1111/cpr.12298. Epub 2016 Sep 14.

Low-intensity pulsed ultrasound upregulates pro-myelination indicators of Schwann cells enhanced by co-culture with adipose-derived stem cells.

Yue Y1,2, Yang X3,4, Zhang L1,5, Xiao X1,2, Nabar NR6, Lin Y1, Hao L1, Zhang D1,2, Huo J1,2, Li J1,2, Cai X1,5, Wang M7,8.

Author information

1
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
2
Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu, China.
3
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China. 65167950@qq.com.
4
Oral Implant Center, West China School of Stomatology, Sichuan University, Chengdu, China. 65167950@qq.com.
5
Oral Implant Center, West China School of Stomatology, Sichuan University, Chengdu, China.
6
Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.
7
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China. hxkqwangm@163.com.
8
Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu, China. hxkqwangm@163.com.

Abstract

OBJECTIVES:

Peripheral nerve injuries are a common occurrence, resulting in considerable patient suffering; it also represents a major economic burden on society. To improve treatment options following peripheral nerve injuries, scientists aim to find a way to promote Schwann cell (SC) myelination to help nerves to carry out their functions effectively. In this study, we investigated myelination ability of SCs, regulated by co-culture with adipose-derived stem cells (ASCs) or low-intensity pulsed ultrasound (LIPUS), and synergistic effects of combined treatments.

MATERIALS AND METHODS:

Schwann cells were co-cultured with or without ASCs, and either left untreated or treated with LIPUS for 10 min/d for 1, 4 or 7 days. Effects of LIPUS and ASC co-culture on pro-myelination indicators of SCs were analysed by real-time PCR (RT-PCR), Western blotting and immunofluorescence staining (IF).

RESULTS:

Our results indicate that ASC-SC co-culture and LIPUS, together or individually, promoted mRNA levels of epidermal growth factor receptor 3 (EGFR3/ErbB3), neuregulin1 (NRG1), early growth response protein 2 (Egr2/Krox20) and myelin basic protein (MBP), with corresponding increases in protein levels of ErbB3, NRG1 and Krox20. Interestingly, combination of ASC-SC co-culture and LIPUS displayed the most remarkable effects.

CONCLUSION:

We demonstrated that ASCs upregulated pro-myelination indicators of SCs by indirect contact (through co-culture) and that effects could be potentiated by LIPUS. We conclude that LIPUS, as a mechanical stress, may have potential in nerve regeneration with potential clinical relevance.

PMID:
27625295
DOI:
10.1111/cpr.12298
[Indexed for MEDLINE]

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