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Sci Rep. 2016 Sep 14;6:33437. doi: 10.1038/srep33437.

High-throughput sequencing-based analysis of endogenetic fungal communities inhabiting the Chinese Cordyceps reveals unexpectedly high fungal diversity.

Author information

1
Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, and Engineering Research Center of Cell &Therapeutic Antibody (Ministry of Education), and School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
2
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, People's Republic of China.
3
Department of Immunology, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
4
Grassland Research Institute, Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai Xining 810016, People's Republic of China.

Abstract

Chinese Cordyceps, known in Chinese as "DongChong XiaCao", is a parasitic complex of a fungus (Ophiocordyceps sinensis) and a caterpillar. The current study explored the endogenetic fungal communities inhabiting Chinese Cordyceps. Samples were collected from five different geographical regions of Qinghai and Tibet, and the nuclear ribosomal internal transcribed spacer-1 sequences from each sample were obtained using Illumina high-throughput sequencing. The results showed that Ascomycota was the dominant fungal phylum in Chinese Cordyceps and its soil microhabitat from different sampling regions. Among the Ascomycota, 65 genera were identified, and the abundant operational taxonomic units showed the strongest sequence similarity to Ophiocordyceps, Verticillium, Pseudallescheria, Candida and Ilyonectria Not surprisingly, the genus Ophiocordyceps was the largest among the fungal communities identified in the fruiting bodies and external mycelial cortices of Chinese Cordyceps. In addition, fungal communities in the soil microhabitats were clustered separately from the external mycelial cortices and fruiting bodies of Chinese Cordyceps from different sampling regions. There was no significant structural difference in the fungal communities between the fruiting bodies and external mycelial cortices of Chinese Cordyceps. This study revealed an unexpectedly high diversity of fungal communities inhabiting the Chinese Cordyceps and its microhabitats.

PMID:
27625176
PMCID:
PMC5022047
DOI:
10.1038/srep33437
[Indexed for MEDLINE]
Free PMC Article

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