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Nat Commun. 2016 Sep 14;7:12674. doi: 10.1038/ncomms12674.

A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells.

Author information

1
CNRS, UMR 6290, Rennes 35043, France.
2
Université de Rennes 1, Institut de Génétique et Développement de Rennes, Rennes 35043, France.
3
Equipe labéllisée Ligue Contre Le Cancer 2014-2016, Rennes 35043, France.
4
Microscopy Rennes Imaging Centre, Biosit, Université de Rennes 1, Rennes 35043, France.

Abstract

Overexpression of AURKA is a major hallmark of epithelial cancers. It encodes the multifunctional serine/threonine kinase aurora A, which is activated at metaphase and is required for cell cycle progression; assessing its activation in living cells is mandatory for next-generation drug design. We describe here a Förster's resonance energy transfer (FRET) biosensor detecting the conformational changes of aurora kinase A induced by its autophosphorylation on Thr288. The biosensor functionally replaces the endogenous kinase in cells and allows the activation of the kinase to be followed throughout the cell cycle. Inhibiting the catalytic activity of the kinase prevents the conformational changes of the biosensor. Using this approach, we discover that aurora kinase A activates during G1 to regulate the stability of microtubules in cooperation with TPX2 and CEP192. These results demonstrate that the aurora kinase A biosensor is a powerful tool to identify new regulatory pathways controlling aurora kinase A activation.

PMID:
27624869
PMCID:
PMC5027284
DOI:
10.1038/ncomms12674
[Indexed for MEDLINE]
Free PMC Article

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