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Dev Cell. 2016 Sep 12;38(5):463-77. doi: 10.1016/j.devcel.2016.08.006.

A Nucleoporin Docks Protein Phosphatase 1 to Direct Meiotic Chromosome Segregation and Nuclear Assembly.

Author information

1
Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0653, USA.
2
Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France.
3
Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0653, USA. Electronic address: abdesai@ucsd.edu.

Abstract

During M-phase entry in metazoans with open mitosis, the concerted action of mitotic kinases disassembles nuclei and promotes assembly of kinetochores-the primary microtubule attachment sites on chromosomes. At M-phase exit, these major changes in cellular architecture must be reversed. Here, we show that the conserved kinetochore-localized nucleoporin MEL-28/ELYS docks the catalytic subunit of protein phosphatase 1 (PP1c) to direct kinetochore disassembly-dependent chromosome segregation during oocyte meiosis I and nuclear assembly during the transition from M phase to interphase. During oocyte meiosis I, MEL-28-PP1c disassembles kinetochores in a timely manner to promote elongation of the acentrosomal spindles that segregate homologous chromosomes. During nuclear assembly, MEL-28 recruits PP1c to the periphery of decondensed chromatin, where it directs formation of a functional nuclear compartment. Thus, a pool of phosphatase activity associated with a kinetochore-localized nucleoporin contributes to two key events that occur during M-phase exit in metazoans: kinetochore disassembly and nuclear reassembly.

KEYWORDS:

ELYS; MEL-28; PP1 docking; centromere; chromosome segregation; kinetochore; meiosis; nuclear pore; nuclear pore assembly; protein phosphatase 1

PMID:
27623381
PMCID:
PMC5094371
DOI:
10.1016/j.devcel.2016.08.006
[Indexed for MEDLINE]
Free PMC Article

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