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EMBO Rep. 2016 Nov;17(11):1565-1577. Epub 2016 Sep 12.

Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor.

Author information

1
Fundación Instituto Leloir - IIBBA CONICET, Buenos Aires, Argentina hbonomi@leloir.org.ar avojnov@fundacioncassara.org.ar fmalamud@iibintech.com.ar.
2
Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, CONICET, Buenos Aires, Argentina.
3
Fundación Instituto Leloir - IIBBA CONICET, Buenos Aires, Argentina.
4
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
5
Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, CONICET, Buenos Aires, Argentina hbonomi@leloir.org.ar avojnov@fundacioncassara.org.ar fmalamud@iibintech.com.ar.
6
UNSAM Campus Miguelete IIB - Instituto de Investigaciones Biotecnológicas, Buenos Aires, Argentina.

Abstract

Phytochromes constitute a major photoreceptor family found in plants, algae, fungi, and prokaryotes, including pathogens. Here, we report that Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease which affects cruciferous crops worldwide, codes for a functional bacteriophytochrome (XccBphP). XccBphP possesses an N-terminal PAS2-GAF-PHY photosensory domain triad and a C-terminal PAS9 domain as its output module. Our results show that illumination of Xcc, prior to plant infection, attenuates its virulence in an XccBphP-dependent manner. Moreover, in response to light, XccBphP downregulates xanthan exopolysaccharide production and biofilm formation, two known Xcc virulence factors. Furthermore, the XccbphP null mutant shows enhanced virulence, similar to that of dark-adapted Xcc cultures. Stomatal aperture regulation and callose deposition, both well-established plant defense mechanisms against bacterial pathogens, are overridden by the XccbphP strain. Additionally, an RNA-Seq analysis reveals that far-red light or XccBphP overexpression produces genomewide transcriptional changes, including the inhibition of several Xcc virulence systems. Our findings indicate that Xcc senses light through XccBphP, eliciting bacterial virulence attenuation via downregulation of bacterial virulence factors. The capacity of XccBphP to respond to light both in vitro and in vivo was abolished by a mutation on the conserved Cys13 residue. These results provide evidence for a novel bacteriophytochrome function affecting an infectious process.

KEYWORDS:

bathy‐type phytochrome; infection; plant defenses; transcriptional regulation; virulence factors

PMID:
27621284
PMCID:
PMC5090697
DOI:
10.15252/embr.201541691
[Indexed for MEDLINE]
Free PMC Article

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