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Nucleic Acids Res. 1989 Jul 11;17(13):5115-23.

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.

Author information

1
MilliGen/Biosearch, Division of Millipore, Burlington, MA 01803.

Abstract

A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.

PMID:
2762122
PMCID:
PMC318099
DOI:
10.1093/nar/17.13.5115
[Indexed for MEDLINE]
Free PMC Article

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