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Sci Rep. 2016 Sep 9;6:32871. doi: 10.1038/srep32871.

Insulin Restores an Altered Corneal Epithelium Circadian Rhythm in Mice with Streptozotocin-induced Type 1 Diabetes.

Author information

1
Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, P.R. China.
2
International Ocular Surface Research Centre and Institute of Ophthalmology, Jinan University Medical School, Guangzhou 510632, P.R. China.
3
Department of Medical Images, The Third People's Hospital, Puyang, China.
4
Section of Leukocyte Biology, Department of Pediatrics, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas, USA.

Abstract

The mechanisms of corneal epithelial lesions and delayed wound repair, as well as their association with diabetes mellitus, are critical issues for clinical ophthalmologists. To test whether the diabetic condition alters the circadian rhythm in a mouse cornea and whether insulin can synchronise the corneal clock, we studied the effects of streptozotocin-induced diabetes on the mitosis of epithelial cells, the recruitment of leukocytes to the cornea, and the expression of main core clock genes (Clock, Bmal1, Per2, Cry1, and Rev-erbα) in the corneal epithelium. We also assessed the possible effect of insulin on these modifications. Diabetes downregulated Clock, Bmal1, and Per2 expression, upregulated Cry1 and Rev-erbα expression, reduced corneal epithelial mitosis, and increased leukocyte (neutrophils and γδ T-cells) recruitment to the cornea. Early treatments with insulin partially restored the altered rhythmicity in the diabetic cornea. In conclusion, insulin-dependent diabetes altered the normal rhythmicity of the cornea, and insulin administration had a beneficial effect on restoring normal rhythmicity in the diabetic cornea.

PMID:
27611469
PMCID:
PMC5017193
DOI:
10.1038/srep32871
[Indexed for MEDLINE]
Free PMC Article

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