Format

Send to

Choose Destination
Cell Death Dis. 2016 Sep 8;7(9):e2362. doi: 10.1038/cddis.2016.246.

MiR-182-5p protects inner ear hair cells from cisplatin-induced apoptosis by inhibiting FOXO3a.

Li Y1,2, Li A1,2, Wu J1,2, He Y2,3, Yu H1,2, Chai R4,5, Li H1,2,6,7.

Author information

1
Department of Otorhinolaryngology, Affiliated Eye and ENT Hospital of Fudan University, Shanghai 200031, China.
2
Key Laboratory for Hearing Science, Ministry of Health, Affiliated Eye and ENT Hospital of Fudan University, Shanghai 200031, China.
3
Central Laboratory, Affiliated Eye and ENT Hospital of Fudan University, Shanghai 200031, China.
4
Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
5
Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
6
Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
7
State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.

Abstract

Cisplatin is widely used for chemotherapy of a variety of malignancies. However, the clinical application of cisplatin is hampered by the resultant irreversible hearing loss due to hair cell apoptosis. To date, no practical regimen to resolve this has been developed. Meanwhile, the role of microRNA in protecting hair cells from cisplatin-induced apoptosis in the inner ear has not been extensively investigated. In this study, we monitored miR-183, -96, and -182 turnover in the cochlea during cisplatin treatment in vitro. We found that overexpression of miR-182, but not miR-183 and -96, improved hair cell survival after 3 μM cisplatin treatment in vitro. We demonstrated that overexpression of miR-182 repressed the intrinsic apoptotic pathway by inhibiting the translation of FOXO3a. Our study offers a new therapeutic target for alleviating cisplatin-induced hair cell apoptosis in a rapid and tissue-specific manner.

PMID:
27607577
PMCID:
PMC5059852
DOI:
10.1038/cddis.2016.246
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center