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Genetics. 2016 Nov;204(3):987-997. doi: 10.1534/genetics.116.191494. Epub 2016 Sep 7.

Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans.

Author information

1
Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, California 95064.
2
Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, California 95064 nbhalla@ucsc.edu.

Abstract

Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes, and is essential for proper meiotic chromosome segregation. In Caenorhabditis elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on pairing centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bona fide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint.

KEYWORDS:

checkpoint; chromosome; meiosis; synapsis; synaptonemal complex

PMID:
27605049
PMCID:
PMC5105873
DOI:
10.1534/genetics.116.191494
[Indexed for MEDLINE]
Free PMC Article

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