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Appl Biochem Biotechnol. 2017 Feb;181(2):573-583. doi: 10.1007/s12010-016-2232-3. Epub 2016 Sep 7.

Fumaric Acid Production from Alkali-Pretreated Corncob by Fed-Batch Simultaneous Saccharification and Fermentation Combined with Separated Hydrolysis and Fermentation at High Solids Loading.

Li X1,2,3, Zhou J4, Ouyang S4, Ouyang J5,6,4, Yong Q5,6,4.

Author information

1
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China. xli@njfu.edu.cn.
2
Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China. xli@njfu.edu.cn.
3
College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China. xli@njfu.edu.cn.
4
College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
5
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.
6
Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.

Abstract

Production of fumaric acid from alkali-pretreated corncob (APC) at high solids loading was investigated using a combination of separated hydrolysis and fermentation (SHF) and fed-batch simultaneous saccharification and fermentation (SSF) by Rhizopus oryzae. Four different fermentation modes were tested to maximize fumaric acid concentration at high solids loading. The highest concentration of 41.32 g/L fumaric acid was obtained from 20 % (w/v) APC at 38 °C in the combined SHF and fed-batch SSF process, compared with 19.13 g/L fumaric acid in batch SSF alone. The results indicated that a combination of SHF and fed-batch SSF significantly improved production of fumaric acid from lignocellulose by R. oryzae than that achieved with batch SSF at high solids loading.

KEYWORDS:

Alkali-pretreated corncob; Fed-batch simultaneous saccharification and fermentation; Fumaric acid; High solids loading; Rhizopus oryzae

PMID:
27604834
DOI:
10.1007/s12010-016-2232-3
[Indexed for MEDLINE]

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