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J Neurooncol. 2017 Jan;131(1):93-101. doi: 10.1007/s11060-016-2272-0. Epub 2016 Sep 7.

In vivo molecular profiling of human glioma using diffusion kurtosis imaging.

Author information

1
Department of Neuroradiology, Eberhard Karls University, Tübingen, Germany. johann-martin.hempel@uni-tuebingen.de.
2
Department of Neuroradiology, Eberhard Karls University, Tübingen, Germany.
3
Department of Neuroradiology, National Hospital of Neurology and Neurosurgery, University College London Hospitals, London, UK.
4
Institute of Neuropathology, Eberhard Karls University, Tübingen, Germany.
5
Department of Neurosurgery, Eberhard Karls University, Tübingen, Germany.
6
Centre of Neurooncology, Comprehensive Cancer Center Tübingen-Stuttgart, Eberhard Karls University, Tübingen, Germany.
7
Department of Preclinical Imaging and Radiopharmacy, Werner Siemens Imaging Center, Eberhard Karls University, Tübingen, Germany.

Abstract

The purpose of this study is to assess the diagnostic performance of diffusion kurtosis imaging (DKI) for in vivo molecular profiling of human glioma. Normalized mean kurtosis (MKn) and mean diffusivity (MDn) metrics from DKI were assessed in 50 patients with histopathologically confirmed glioma. The results were compared in regard to the WHO-based histological findings and molecular characteristics leading to integrated diagnosis (Haarlem Consensus): isocitrate-dehydrogenase (IDH1/2) mutation status, alpha-thalassemia/mental retardation syndrome X-linked (ATRX) expression, chromosome 1p/19q loss of heterozygosity (LOH), and O6-methylguanine DNA methyltransferase (MGMT) promoter methylation status. MKn was significantly lower in tumors with IDH1/2 mutation (0.43 ± 0.09) and ATRX loss of expression (0.41 ± 0.11) than in those with IDH1/2 wild type (0.57 ± 0.09, p < 0.001) and ATRX maintained expression (0.51 ± 0.10, p = 0.004), respectively. Regarding the integrated molecular diagnosis, MKn was significantly higher in primary glioblastoma (0.57 ± 0.10) than in astrocytoma (0.39 ± 0.11, p < 0.001) and oligodendroglioma (0.47 ± 0.05, p = 0.003). MK may be used to provide insight into the human glioma molecular profile regarding IDH1/2 mutation status and ATRX expression. Considering the diagnostic and prognostic significance of these molecular markers, MK appears to be a promising in vivo biomarker for glioma. The diagnostic performance of MK seems to fit more with the integrated molecular approach than the conventional histological findings of the current WHO 2007 classification.

KEYWORDS:

1p/19q LOH; ATRX; Diffusion kurtosis imaging; Glioma; Haarlem consensus; IDH1/2; Integrated diagnosis; Isocitrate dehydrogenase; MGMT

PMID:
27604789
DOI:
10.1007/s11060-016-2272-0
[Indexed for MEDLINE]

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