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Microarrays (Basel). 2015 Apr 24;4(2):214-27. doi: 10.3390/microarrays4020214.

NAPPA as a Real New Method for Protein Microarray Generation.

Author information

1
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. pauladg@usal.es.
2
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. pauladg@usal.es.
3
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mariagg@usal.es.
4
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mariagg@usal.es.
5
Rheumatology Division, ProteoRed/ISCIII Proteomics Group, INIBIC, Hospital Universitario de A Coruña, A Coruña 15006, Spain. llourido@udc.es.
6
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. romade@usal.es.
7
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. nibarrola@usal.es.
8
Biotechnology National Centre, Spanish National Research Council (CSIC), Madrid 28049, Spain. jcasado@cnb.csic.es.
9
Biodesign Institute, Arizona State University, 1001 South McAllister Avenue, Tempe, AZ 85287, USA. Joshua.Labaer@asu.edu.
10
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mfuentes@usal.es.
11
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mfuentes@usal.es.

Abstract

Nucleic Acid Programmable Protein Arrays (NAPPA) have emerged as a powerful and innovative technology for the screening of biomarkers and the study of protein-protein interactions, among others possible applications. The principal advantages are the high specificity and sensitivity that this platform offers. Moreover, compared to conventional protein microarrays, NAPPA technology avoids the necessity of protein purification, which is expensive and time-consuming, by substituting expression in situ with an in vitro transcription/translation kit. In summary, NAPPA arrays have been broadly employed in different studies improving knowledge about diseases and responses to treatments. Here, we review the principal advances and applications performed using this platform during the last years.

KEYWORDS:

NAPPA; biomarker; high-throughput screening; microarray generation; protein microarray; protein-protein interaction

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