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PLoS One. 2016 Sep 6;11(9):e0161914. doi: 10.1371/journal.pone.0161914. eCollection 2016.

Global Profiling of the Cellular Alternative RNA Splicing Landscape during Virus-Host Interactions.

Author information

1
Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Quebec, J1E 4K8, Canada.
2
Laboratoire de Génomique Fonctionnelle, Université de Sherbrooke, Sherbrooke, Quebec, J1E 4K8, Canada.
3
Département de microbiologie, infectiologie et immunologie, Faculté de médecine, Université de Montréal, Montreal, Quebec, H3C 3J7, Canada.

Abstract

Alternative splicing (AS) is a central mechanism of genetic regulation which modifies the sequence of RNA transcripts in higher eukaryotes. AS has been shown to increase both the variability and diversity of the cellular proteome by changing the composition of resulting proteins through differential choice of exons to be included in mature mRNAs. In the present study, alterations to the global RNA splicing landscape of cellular genes upon viral infection were investigated using mammalian reovirus as a model. Our study provides the first comprehensive portrait of global changes in the RNA splicing signatures that occur in eukaryotic cells following infection with a human virus. We identify 240 modified alternative splicing events upon infection which belong to transcripts frequently involved in the regulation of gene expression and RNA metabolism. Using mass spectrometry, we also confirm modifications to transcript-specific peptides resulting from AS in virus-infected cells. These findings provide additional insights into the complexity of virus-host interactions as these splice variants expand proteome diversity and function during viral infection.

PMID:
27598998
PMCID:
PMC5012649
DOI:
10.1371/journal.pone.0161914
[Indexed for MEDLINE]
Free PMC Article

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