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Mitochondrion. 2017 Mar;33:84-101. doi: 10.1016/j.mito.2016.08.017. Epub 2016 Aug 31.

The mitochondrial BKCa channel cardiac interactome reveals BKCa association with the mitochondrial import receptor subunit Tom22, and the adenine nucleotide translocator.

Author information

1
Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Molecular & Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA.
2
Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
3
Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Physiology, University of California, Los Angeles, Los Angeles, CA, USA; Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA; Cardiovascular Research Laboratory, University of California, Los Angeles, Los Angeles, CA, USA.
4
Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Molecular & Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA; Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA; Cardiovascular Research Laboratory, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address: ltoro@ucla.edu.

Abstract

Mitochondrial BKCa channel, mitoBKCa, regulates mitochondria function in the heart but information on its protein partnerships in cardiac mitochondria is missing. A directed proteomic approach discovered the novel interaction of BKCa with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). The expressed protein partners co-immunoprecipitated and co-segregated into mitochondrial fractions in HEK293T cells. The BKCa 50 amino acid splice insert, DEC, facilitated BKCa interaction with ANT. Further, BKCa transmembrane domain was required for the association with both Tom22 and ANT. The results serve as a working framework to understand mitoBKCa import and functional relationships.

KEYWORDS:

Adenine nucleotide translocator; BK channel; Heart; Macromolecular complex; MaxiK channel; Mitochondria; Potassium ion channel; Proteomics; Tom22

PMID:
27592226
PMCID:
PMC5332438
DOI:
10.1016/j.mito.2016.08.017
[Indexed for MEDLINE]
Free PMC Article

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