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Talanta. 2016 Nov 1;160:425-430. doi: 10.1016/j.talanta.2016.07.049. Epub 2016 Jul 25.

Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis.

Author information

1
Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China.
2
Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China. Electronic address: niyi20042004@hotmail.com.
3
Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka, Suita-city, Osaka, 565-0871 Japan.
4
Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Yamadaoka, Suita-city, Osaka, 565-0871 Japan.
5
School of Optoelectronic Engineering, ChangZhou Institute of Technology, 299 South Tongjiang Road, Changzhou, Jiangsu, 213002 China.
6
Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka, Suita-city, Osaka, 565-0871 Japan; School of Optoelectronic Engineering, ChangZhou Institute of Technology, 299 South Tongjiang Road, Changzhou, Jiangsu, 213002 China. Electronic address: xmdou@ecust.edu.cn.

Abstract

High-speed capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95s via present short-end CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R(2) and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098ng/μl, respectively.

KEYWORDS:

Oral bacteria; Polymerase chain reaction; Short-end capillary electrophoresis; Space domain internal standard method

PMID:
27591633
DOI:
10.1016/j.talanta.2016.07.049
[Indexed for MEDLINE]

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