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J Biomed Mater Res A. 2017 Jul;105(7):2020-2034. doi: 10.1002/jbm.a.35890. Epub 2017 May 10.

Gelatin/chondroitin sulfate nanofibrous scaffolds for stimulation of wound healing: In-vitro and in-vivo study.

Author information

1
Tissue Engineering and Regenerative Medicine Institute, Tehran Central Branch, Islamic Azad University, Tehran, Iran.
2
Department of Biomaterials, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/159, Tehran, Iran.
3
Department of Polymer Engineering & Color Technology, Amirkabir University of Technology, P.O. Box: 15875/4413, Tehran, Iran.
4
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, , P.O. Box 19395/4644, Tehran, Iran.
5
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
6
Department of Regenerative Medicine at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box 19395/4644, Tehran, Iran.
7
Department of Bioengineering, Molecular Cell Biomechanics Laboratory, University of California Berkeley, 208A Stanley Hall, Berkeley, California.
8
Department of Mechanical Engineering, Molecular Cell Biomechanics Laboratory, University of California Berkeley, 208A Stanley Hall, Berkeley, California.

Abstract

In this research, fabrication of gelatin/chondroitin sulfate (GAG) nanofibrous scaffolds using electrospinning technique for skin tissue engineering was studied. The influence of GAG content on chemical, physical, mechanical and biological properties of the scaffolds were investigated. Human dermal fibroblast (HDF) cells were cultured and bioactivity of electrospun gelatin/GAG scaffolds for skin tissue engineering was assayed. Biological results illustrated that HDF cells attached and spread well on gelatin/GAG nanofibrous scaffolds displaying spindle-like shapes and stretching. MTS assay was performed to evaluate the cell proliferation on electrospun gelatin/GAG scaffolds. The results confirmed the influence of GAG content as well as the nanofibrous structure on cell proliferation and attachment of substrates. The gelatin/GAG nanofibrous scaffolds with the desired thickness for in-vivo evaluations were used on the full-thickness wounds. Pathobiological results showed that cell loaded gelatin/GAG scaffolds significantly accelerated wounds healing.

KEYWORDS:

chondroitin sulfate; gelatin; in vivo; nanofibrous; skin; wound healing

PMID:
27588562
DOI:
10.1002/jbm.a.35890
[Indexed for MEDLINE]

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