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Cold Spring Harb Protoc. 2016 Sep 1;2016(9). doi: 10.1101/pdb.prot090795.

Detection of Indel Mutations in Drosophila by High-Resolution Melt Analysis (HRMA).

Author information

1
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115; Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115.

Abstract

Although CRISPR technology allows specific genome alterations to be created with relative ease, detection of these events can be problematic. For example, CRISPR-induced double-strand breaks are often repaired imprecisely to generate unpredictable short indel mutations. Detection of these events requires the use of molecular screening techniques such as endonuclease assays, restriction profiling, or high-resolution melt analysis (HRMA). Here, we provide detailed protocols for HRMA-based mutation screening in Drosophila and analysis of the resulting data using the online tool HRMAnalyzer.

PMID:
27587781
DOI:
10.1101/pdb.prot090795
[Indexed for MEDLINE]

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