Format

Send to

Choose Destination
Chin J Integr Med. 2016 Sep 1. [Epub ahead of print]

Prunella vulgaris polysaccharide inhibits growth and migration of breast carcinoma-associated fibroblasts by suppressing expression of basic fibroblast growth factor.

Hao J1,2,3, Ding XL1,2,3, Yang X1,2,3, Wu XZ4,5,6.

Author information

1
Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
2
National Clinical Research Center for Cancer, Tianjin, China.
3
Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
4
Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China. wuxiongzhi@163.com.
5
National Clinical Research Center for Cancer, Tianjin, China. wuxiongzhi@163.com.
6
Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China. wuxiongzhi@163.com.

Abstract

OBJECTIVE:

To study the effects of Prunella vulgaris polysaccharide (PVP) on human breast carcinoma-associated fibroblasts (CAFs).

METHODS:

Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)-2H-tetrazolium (MTS) assay. Wound healing experiment and transwell migration assay were used to investigate the anti-migration effects. Flow cytometry was applied to detect cell apoptosis and cell cycle distribution. Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fifibroblast growth factor (bFGF) in CAFs. Culture SKBr-3 with CAFs conditioned medium (CAFs-CM) to evaluate the indirect function on the proliferation of breast cancer SKBr-3 cells.

RESULTS:

PVP inhibited the viability of CAFs by inducing apoptosis (P <0.01) and arresting cell cycle (P <0.01). It also inhibited the migration of CAFs (P <0.01). bFGF promoted CAFs proliferation (P <0.01) and migration (P <0.01), protected CAFs from apoptosis (P <0.05) and reduced G0 phase to 49.06% (P <0.01). However, these effects of bFGF on CAFs could be abrogated by PVP. Culturing SKBr-3 with CAFs-CM, PVP could inhibit the viability of breast cancer SKBr-3 cells indirectly. Moreover, PVP reduced the mRNA expression (P <0.01) and protein secretion of bFGF (P <0.01) in CAFs.

CONCLUSION:

PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expression, thus inhibiting the growth of breast cancer SKBr-3 cells indirectly.

KEYWORDS:

Chinese medicine; Prunella vulgaris polysaccharide; SKBr-3; basic fibroblast growth factor; carcinoma-associated fibroblasts

PMID:
27586473
DOI:
10.1007/s11655-016-2587-x

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center