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Genetics. 2016 Nov;204(3):1151-1159. doi: 10.1534/genetics.116.192898. Epub 2016 Aug 31.

The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis elegans Locomotion.

Chen D1,2, Taylor KP1,2,3, Hall Q1,2, Kaplan JM4,2,3.

Author information

1
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.
2
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115.
3
Biological and Biomedical Sciences Program, Harvard Medical School, Boston, Massachusetts 02115.
4
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 Kaplan@molbio.mgh.harvard.edu.

Abstract

During larval molts, Caenorhabditis elegans exhibits a sleep-like state (termed lethargus) that is characterized by the absence of feeding and profound locomotion quiescence. The rhythmic pattern of locomotion quiescence and arousal linked to the molting cycle is mediated by reciprocal changes in sensory responsiveness, whereby arousal is associated with increased responsiveness. Sensory neurons arouse locomotion via release of a neuropeptide (PDF-1) and glutamate. Here we identify a second arousing neuropeptide (FLP-2). We show that FLP-2 acts via an orexin-like receptor (FRPR-18), and that FLP-2 and PDF-1 secretion are regulated by reciprocal positive feedback. These results suggest that the aroused behavioral state is stabilized by positive feedback between two neuropeptides.

KEYWORDS:

Caenorhabditis elegans; PDF; lethargus; orexin; sleep

PMID:
27585848
PMCID:
PMC5105848
DOI:
10.1534/genetics.116.192898
[Indexed for MEDLINE]
Free PMC Article

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