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Methods Mol Biol. 2016;1470:103-19. doi: 10.1007/978-1-4939-6337-9_9.

In Vitro-Pooled shRNA Screening to Identify Determinants of Radiosensitivity.

Author information

1
Adaptimmune Ltd, 91 Park Drive, Milton Park, Oxon, OX14 4RY, UK. alessandro_ceroni@hotmail.com.
2
Department of Oncology, University of Oxford-Old Road Campus, Oxford, UK.
3
Target Discovery Institute, Nuffield Department of Medicine, University of Oxford-Old Road Campus, Oxford, UK.

Abstract

Short hairpin RNA (shRNA)-pooled screening is a valuable and cost-effective tool for assaying the contribution of individual genes to cell viability and proliferation on a genomic scale. Here we describe the key considerations for the design and execution of a pooled shRNA screen to identify determinants of radiosensitivity.

KEYWORDS:

Genomic screening; Lentivirus; Pooled library; Radiosensitivity; shRNA

PMID:
27581288
DOI:
10.1007/978-1-4939-6337-9_9
[Indexed for MEDLINE]

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