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Methods Mol Biol. 2016;1458:179-93. doi: 10.1007/978-1-4939-3801-8_13.

Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.

Author information

1
Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, 91120, Jerusalem, Israel.
2
Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, 91120, Jerusalem, Israel. zvikag@ekmd.huji.ac.il.

Abstract

Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.

KEYWORDS:

Cancer; Cytotoxicity; Density; Enrichment; Flow cytometry; Isolation; Neutrophils

PMID:
27581022
DOI:
10.1007/978-1-4939-3801-8_13
[Indexed for MEDLINE]

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