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Nat Commun. 2016 Sep 1;7:12410. doi: 10.1038/ncomms12410.

Virus-derived DNA drives mosquito vector tolerance to arboviral infection.

Author information

1
Institut Pasteur, Viruses and RNA Interference Unit, Centre National de la Recherche Scientifique UMR 3569, 75724 Paris cedex 15, France.
2
Institut Pasteur, Viral Populations and Pathogenesis Unit, Centre National de la Recherche Scientifique UMR 3569, 75724 Paris cedex 15, France.
3
IRCAN, INSERM U1081, Centre National de la Recherche Scientifique UMR 7284, University of Nice-Sophia-Antipolis, 06107 Nice Cedex 2, France.
4
FHU OncoAge, University of Nice-Sophia Antipolis, 06107 Nice, France.
5
Institut Pasteur, Insect-Virus Interactions Group, Centre National de la Recherche Scientifique URA 3012, 75724 Paris cedex 15, France.

Abstract

Mosquitoes develop long-lasting viral infections without substantial deleterious effects, despite high viral loads. This makes mosquitoes efficient vectors for emerging viral diseases with enormous burden on public health. How mosquitoes resist and/or tolerate these viruses is poorly understood. Here we show that two species of Aedes mosquitoes infected with two arboviruses from distinct families (dengue or chikungunya) generate a viral-derived DNA (vDNA) that is essential for mosquito survival and viral tolerance. Inhibition of vDNA formation leads to extreme susceptibility to viral infections, reduction of viral small RNAs due to an impaired immune response, and loss of viral tolerance. Our results highlight an essential role of vDNA in viral tolerance that allows mosquito survival and thus may be important for arbovirus dissemination and transmission. Elucidating the mechanisms of mosquito tolerance to arbovirus infection paves the way to conceptualize new antivectorial strategies to selectively eliminate arbovirus-infected mosquitoes.

PMID:
27580708
PMCID:
PMC5025746
DOI:
10.1038/ncomms12410
[Indexed for MEDLINE]
Free PMC Article

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