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BMC Complement Altern Med. 2016 Aug 30;16(1):325. doi: 10.1186/s12906-016-1327-2.

Investigation of the Immunomodulatory effect of Berberis vulgaris on core-pulsed dendritic cell vaccine.

Author information

1
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
2
Center for Aging and Associated Diseases, Helmy Institute for Medical Sciences, Zewail City, Egypt.
3
Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
4
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt. minasaad@windowslive.com.
5
Biomedical Technology, GEBRI, SRTA-city, Alexandria, Egypt.

Abstract

BACKGROUND:

Virus-induced dendritic cells (DCs) functional deficiency leads to sub-optimal initiation of adaptive immune responses and consequently chronic infection establishment. The present study reports an advanced hepatitis C virus (HCV) therapeutic vaccine model based on In vivo enrichment of DCs with barberry ethanolic crude extract (BCE) then pulsing them with HCV core protein.

METHODS:

DCs were enriched by BCE intravenous injection in BALB/c mice. Vaccine efficiency was assessed by flow cytometric analysis of splenocytes of immunized mice, cytokine profiling, cytotoxic T lymphocyte assay, and humoral immune response assessment.

RESULTS:

There was no significant difference in surface phenotypic characterization of splenocytes from mice immunized with non-BCE-enriched-core-pulsed DCs (iDcs-core) compared to those from mice injected with RPMI-1640 medium. However, splenocytes from mice immunized with BCE-enriched-core-pulsed DCs showed 197 % increase in CD16+ population, 33 % increase in MHCII(+) population, and 43 % decrease in CD3(+) population. In iDCs-core group, 57.9 % greater anti-core cytotoxic T lymphocyte activity, up-regulation in interferon gamma and interleukin (IL) -12 expression, and down-regulation in IL-4 and IL-10 were recorded. Moreover, sustained specific anti-core antibodies were detected only in sera of the same group.

CONCLUSIONS:

results indicate that BCE-enriched-core-transduced DCs may serve as a new model for immunotherapy of HCV chronic infection.

KEYWORDS:

Berberis vulgaris; IL 12; IL 4; INF-γ; Th-1

PMID:
27577059
PMCID:
PMC5004255
DOI:
10.1186/s12906-016-1327-2
[Indexed for MEDLINE]
Free PMC Article

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