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Biochem Biophys Res Commun. 1989 Jul 31;162(2):761-6.

The measurement and mass spectral identification of indole-3-pyruvate from tomato shoots.

Author information

1
Department of Biochemistry, Auckland University, New Zealand.

Abstract

Endogenous indole-3-pyruvate has been identified by full-scan combined gas chromatography-mass spectrometry and measured using gas chromatography with an electron capture detector. High specific-activity [5-3H]indole-3-pyruvate was synthesized from [5-3H]tryptophan and used as an internal standard. In order to allow purification of the labile indole-3-pyruvate it was stabilised by the formation in the crude extract of its pentafluorobenzyl oxime derivative. This derivative also allowed sensitive detection and measurement of indole-3-pyruvate in the picogram range using a gas chromatograph with an electron capture detector. Endogenous levels were found to be between 8-10 ng/g f.wt. of tomato shoots which is comparable to that of the indole-3-acetic acid pool size, 11 ng/g f.wt., in this tissue.

PMID:
2757639
DOI:
10.1016/0006-291x(89)92375-9
[Indexed for MEDLINE]

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