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Nat Microbiol. 2016 May 27;1(7):16069. doi: 10.1038/nmicrobiol.2016.69.

Influenza virus mRNA trafficking through host nuclear speckles.

Author information

1
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9039, USA.
2
Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6059, USA.
3
Department of Microbiology, New York, New York 10029, USA.
4
Global Health and Emerging Pathogens Institute, New York, New York 10029, USA.
5
Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
6
Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.

Abstract

Influenza A virus is a human pathogen with a genome composed of eight viral RNA segments that replicate in the nucleus. Two viral mRNAs are alternatively spliced. The unspliced M1 mRNA is translated into the matrix M1 protein, while the ion channel M2 protein is generated after alternative splicing. These proteins are critical mediators of viral trafficking and budding. We show that the influenza virus uses nuclear speckles to promote post-transcriptional splicing of its M1 mRNA. We assign previously unknown roles for the viral NS1 protein and cellular factors to an intranuclear trafficking pathway that targets the viral M1 mRNA to nuclear speckles, mediates splicing at these nuclear bodies and exports the spliced M2 mRNA from the nucleus. Given that nuclear speckles are storage sites for splicing factors, which leave these sites to splice cellular pre-mRNAs at transcribing genes, we reveal a functional subversion of nuclear speckles to promote viral gene expression.

KEYWORDS:

influenza virus; mRMA export; nuclear speckles; splicing

PMID:
27572970
PMCID:
PMC4917225
DOI:
10.1038/nmicrobiol.2016.69
[Indexed for MEDLINE]
Free PMC Article

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