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Nat Microbiol. 2016 May 23;1(6):16058. doi: 10.1038/nmicrobiol.2016.58.

Selection of antigenically advanced variants of seasonal influenza viruses.

Author information

1
Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
2
Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
3
World Health Organization Collaborating Centre for Modeling, Evolution, and Control of Emerging Infectious Diseases, Cambridge CB2 3EJ, UK.
4
Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
5
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan.
6
ERATO Infection-Induced Host Responses Project, Saitama 332-0012, Japan.
7
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
8
Fogarty International Center, National Institutes of Health, Bethesda, MD 20892, USA.
9
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
10
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA.
11
WHO Collaborating Centre for Reference and Research on Influenza (VIDRL) at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
12
Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
13
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
14
Influenza Virus Research Center, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan.
15
Department of Virology, Erasmus Medical Center, 3000 CA Rotterdam, Netherlands.
#
Contributed equally

Abstract

Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. We also selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014-2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature.

PMID:
27572841
PMCID:
PMC5087998
DOI:
10.1038/nmicrobiol.2016.58
[Indexed for MEDLINE]
Free PMC Article

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