Format

Send to

Choose Destination
Methods Enzymol. 2016;579:125-57. doi: 10.1016/bs.mie.2016.04.012. Epub 2016 May 31.

Processing of Structurally Heterogeneous Cryo-EM Data in RELION.

Author information

1
MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, United Kingdom. Electronic address: scheres@mrc-lmb.cam.ac.uk.

Abstract

This chapter describes algorithmic advances in the RELION software, and how these are used in high-resolution cryo-electron microscopy (cryo-EM) structure determination. Since the presence of projections of different three-dimensional structures in the dataset probably represents the biggest challenge in cryo-EM data processing, special emphasis is placed on how to deal with structurally heterogeneous datasets. As such, this chapter aims to be of practical help to those who wish to use RELION in their cryo-EM structure determination efforts.

KEYWORDS:

Classification; Cryo-electron microscopy; Image processing; Maximum likelihood; Protein dynamics; RELION; Single-particle analysis; Structural heterogeneity

PMID:
27572726
DOI:
10.1016/bs.mie.2016.04.012
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center