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J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Oct 15;1033-1034:234-241. doi: 10.1016/j.jchromb.2016.08.011. Epub 2016 Aug 11.

Development and validation of a LC-MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma.

Author information

1
EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France. Electronic address: 07silvia@gmail.com.
2
EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France.
3
EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Assistance Publique-Hôpitaux de Paris (APHP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Service de Pharmacologie Clinique, 75014 Paris, France.
4
Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris (APHP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Department of Histology, Embryology and Biology of Reproduction, 75679 Paris, France.
5
Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France.
6
EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France; Assistance Publique-Hôpitaux de Paris (APHP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Service de Pharmacologie Clinique, 75014 Paris, France; CIC-0901 Inserm, Cochin-Necker, 75015 Paris, France.

Abstract

Accurate and sensitive liquid-chromatography tandem mass spectrometry method for the quantification of tenofovir and emtricitabine in seminal plasma has been developed and full validated. Molecules were separated by high-performance liquid chromatography on an Atlantis T3 C18 column using a gradient of deionized water and methanol, including 0.05% formic acid (250μl/min) and detected by electrospray ionisation/tandem mass spectrometry in positive ion mode. The method was validated over a clinical range of 3.13-1000ng/mL for tenofovir and 6.25-2000ng/mL for emtricitabine. Inter and intra-assay precisions were <9.37% for tenofovir and<10.88% for emtricitabine, and accuracies were between 0.48% and 8.43% for tenofovir, and between 0.64% and 13.87% for emtricitabine. The developed method was successfully applied for analysing tenofovir and emtricitabine concentrations in seminal plasma samples from a clinical study. The use of tandem mass spectrometry can be a suitable method for the analysis of this kind of matrices, providing high sensitivity and specificity to the analysis.

KEYWORDS:

Emtricitabine; LC–MS/MS; Seminal plasma; Tenofovir

PMID:
27571683
DOI:
10.1016/j.jchromb.2016.08.011
[Indexed for MEDLINE]

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