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Nat Methods. 2016 Oct;13(10):841-4. doi: 10.1038/nmeth.3965. Epub 2016 Aug 29.

rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome.

Author information

1
Department of Chemistry, University of Cambridge, Cambridge, UK.
2
Cancer Research UK, Cambridge Institute, Cambridge, UK.

Abstract

We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.

PMID:
27571552
DOI:
10.1038/nmeth.3965
[Indexed for MEDLINE]

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