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PLoS One. 2016 Aug 29;11(8):e0161008. doi: 10.1371/journal.pone.0161008. eCollection 2016.

Genomic Analyses of Cladophialophora bantiana, a Major Cause of Cerebral Phaeohyphomycosis Provides Insight into Its Lifestyle, Virulence and Adaption in Host.

Author information

1
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
2
Department of Neurosurgery, Hospital Pulau Pinang, Jalan Residensi, Georgetown, Pulau Pinang, Malaysia.
3
Department of Surgery, Neurosurgical Division, University of Malaya, Kuala Lumpur, Malaysia.
4
Codon Genomics SB, Selangor Darul Ehsan, Malaysia.

Abstract

Cladophialophora bantiana is a dematiaceous fungus with a predilection for causing central nervous system (CNS) infection manifesting as brain abscess in both immunocompetent and immunocompromised patients. In this paper, we report comprehensive genomic analyses of C. bantiana isolated from the brain abscess of an immunocompetent man, the first reported case in Malaysia and Southeast Asia. The identity of the fungus was determined using combined morphological analysis and multilocus phylogeny. The draft genome sequence of a neurotrophic fungus, C. bantiana UM 956 was generated using Illumina sequencing technology to dissect its genetic fundamental and basic biology. The assembled 37.1 Mb genome encodes 12,155 putative coding genes, of which, 1.01% are predicted transposable elements. Its genomic features support its saprophytic lifestyle, renowned for its versatility in decomposing hemicellulose and pectin components. The C. bantiana UM 956 was also found to carry some important putative genes that engaged in pathogenicity, iron uptake and homeostasis as well as adaptation to various stresses to enable the organism to survive in hostile microenvironment. This wealth of resource will further catalyse more downstream functional studies to provide better understanding on how this fungus can be a successful and persistent pathogen in human.

PMID:
27570972
PMCID:
PMC5003357
DOI:
10.1371/journal.pone.0161008
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

Authors YCT, PSC, CCH, and WYY are employed by Codon Genomics SB. The other authors named on the manuscript are from University of Malaya. All other authors have declared that no competing interests exist. These affiliations do not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

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