Format

Send to

Choose Destination
Chem Biol Drug Des. 2017 Mar;89(3):289-296. doi: 10.1111/cbdd.12848. Epub 2016 Sep 28.

Structure-based discovery of novel US28 small molecule ligands with different modes of action.

Author information

1
Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
2
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.
3
Faculty of Chemistry, Alexandru Ioan Cuza University of Iaşi, Iaşi, Romania.
4
Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
5
Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark.
6
Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark.

Abstract

The human cytomegalovirus-encoded G protein-coupled receptor US28 is a constitutively active receptor, which can recognize various chemokines. Despite the recent determination of its 2.9 Å crystal structure, potent and US28-specific tool compounds are still scarce. Here, we used structural information from a refined US28:VUF2274 complex for virtual screening of >12 million commercially available small molecule compounds. Using a combined receptor- and ligand-based approach, we tested 98 of the top 0.1% ranked compounds, revealing novel chemotypes as compared to the ~1.45 million known ligands in the ChEMBL database. Two compounds were confirmed as agonist and inverse agonist, respectively, in both IP accumulation and Ca2+ mobilization assays. The screening setup presented in this work is computationally inexpensive and therefore particularly useful in an academic setting as it enables simultaneous testing in binding as well as in different functional assays and/or species without actual chemical synthesis.

KEYWORDS:

G protein-coupled receptor; drug discovery; molecular modeling; virtual screening

PMID:
27569905
DOI:
10.1111/cbdd.12848
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center