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Biochem Biophys Res Commun. 2016 Sep 23;478(3):1429-35. doi: 10.1016/j.bbrc.2016.08.141. Epub 2016 Aug 25.

Expression and cellular localization of HCN channels in rat cerebellar granule neurons.

Author information

1
Centro de Investigaciones Médicas CIM, Escuela de Medicina, Universidad de Talca, Talca, Chile.
2
Departamento de Fisiología, Universidad de Concepción, Concepción, Chile.
3
Centro de Investigaciones Médicas CIM, Escuela de Medicina, Universidad de Talca, Talca, Chile. Electronic address: lzuniga@utalca.cl.

Abstract

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels belong to the superfamily of voltage-gated pore loop channels. In mammals, this family consists of four different subunits (HCN1-4) and their ion channels activity have been proposed to play an essential role in regulating the membrane potential of excitable cells. Here, we describe the expression and relative abundances of HCN channels in cerebellum and primary cultures of cerebellar granule neurons (CGN). Quantitative determination of mRNA expression levels demonstrated the existence of an accumulation pattern of transcripts in cerebellum that encode HCN2 > HCN3 = HCN4 > HCN1 subunits. Immunolocalization analyses of HCN channels in cerebella revealed positive staining in Purkinje and granule cell layers. The presence of the HCN subunits in the cerebellar granule cell layer was then confirmed in primary cultures of CGN by quantitative real-time PCR (qPCR), as well as western blot and immunofluorescence analysis, demonstrating the presence of all four channel proteins.

KEYWORDS:

Cerebellar granule neurons (CGN); HCN channels; HCN channels in cerebellum; Hyperpolarization-activated cyclic nucleotide-gated channels

PMID:
27569278
DOI:
10.1016/j.bbrc.2016.08.141
[Indexed for MEDLINE]

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