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Immunol Lett. 2016 Oct;178:114-21. doi: 10.1016/j.imlet.2016.08.010. Epub 2016 Aug 26.

Dectin-1 agonist selectively induces IgG1 class switching by LPS-activated mouse B cells.

Author information

1
Department of Microbiology, Myunggok Medical Research Center, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea.
2
Department of Microbiology, Myunggok Medical Research Center, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea. Electronic address: srpark@konyang.ac.kr.

Abstract

Heat-killed Saccharomyces cerevisiae (HKSC) is an agonist for Dectin-1, a major fungal cell wall β-glucan receptor. We previously reported that HKSC selectively enhances IgG1 production by LPS-activated mouse B cells. To determine if this IgG1 selectivity is caused by selective IgG1 class switching, we performed RT-PCRs for measuring germline transcripts (GLTs), flow cytometric analyses for detecting Ig-expressing cells, and ELISPOT assays for measuring the number of Ig-secreting cells in HKSC/LPS-stimulated mouse B cell cultures. HKSC selectively enhanced expression of GLTγ1, the number of IgG1-expressing cells, and the number of IgG1-secreting B cells in the presence of LPS stimulation. In addition, HKSC induced the expression of CD69, an activation marker for B lymphocytes, and the expression of surface Dectin-1. Two Dectin-1 antagonists, laminarin and a neutralizing Dectin-1 antibody, selectively diminished HKSC-reinforced IgG1 production by LPS-stimulated B cells. Furthermore, depleted zymosan (dzn), a Dectin-1 agonist with increased selectivity, also selectively enhanced GLTγ1 transcription. The Dectin-1 antagonists blocked dzn-induced IgG1 production by LPS-activated B cells. Collectively, these results suggest that Dectin-1 agonists selectively induce IgG1 class switching by direct stimulation of Dectin-1 on LPS-activated B cells resulting in selective production of IgG1.

KEYWORDS:

B cells; Dectin-1; Germline γ1 transcripts; IgG1; LPS

PMID:
27568820
DOI:
10.1016/j.imlet.2016.08.010
[Indexed for MEDLINE]

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