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Biotechnol Bioeng. 2016 Oct;113(10):2228-40. doi: 10.1002/bit.25984. Epub 2016 Apr 8.

Plasmonic activation of gold nanorods for remote stimulation of calcium signaling and protein expression in HEK 293T cells.

Author information

1
Department of Chemical and Biological Engineering, and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York, 12180.
2
Laboratory of Molecular Genetics, Rockefeller University, 1230 York Ave, New York, New York, 10065.
3
Department of Materials Science and Engineering, Rensselaer Polytechnic Institute, Troy, New York, 12180.
4
Weill Cornell Medical College, New York, New York.
5
Laboratory of Molecular Genetics, Rockefeller University, 1230 York Ave, New York, New York, 10065. friedj@mail.rockefeller.edu.
6
Department of Chemical and Biological Engineering, and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York, 12180. dordick@rpi.edu.
7
Department of Materials Science and Engineering, Rensselaer Polytechnic Institute, Troy, New York, 12180. dordick@rpi.edu.
8
Departments of Biomedical Engineering and Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, 12180. dordick@rpi.edu.

Abstract

Remote activation of specific cells of a heterogeneous population can provide a useful research tool for clinical and therapeutic applications. Here, we demonstrate that photostimulation of gold nanorods (AuNRs) using a tunable near-infrared (NIR) laser at specific longitudinal surface plasmon resonance wavelengths can induce the selective and temporal internalization of calcium in HEK 293T cells. Biotin-PEG-Au nanorods coated with streptavidin Alexa Fluor-633 and biotinylated anti-His antibodies were used to decorate cells genetically modified with His-tagged TRPV1 temperature-sensitive ion channel and AuNRs conjugated to biotinylated RGD peptide were used to decorate integrins in unmodified cells. Plasmonic activation can be stimulated at weak laser power (0.7-4.0 W/cm(2) ) without causing cell damage. Selective activation of TRPV1 channels could be controlled by laser power between 1.0 and 1.5 W/cm(2) . Integrin targeting robustly stimulated calcium signaling due to a dense cellular distribution of nanoparticles. Such an approach represents a functional tool for combinatorial activation of cell signaling in heterogeneous cell populations. Our results suggest that it is possible to induce cell activation via NIR-induced gold nanorod heating through the selective targeting of membrane proteins in unmodified cells to produce calcium signaling and downstream expression of specific genes with significant relevance for both in vitro and therapeutic applications. Biotechnol. Bioeng. 2016;113: 2228-2240.

KEYWORDS:

TRPV1 ion channel; calcium signaling; combinatorial activation; gold nanorods; integrins

PMID:
27563853
DOI:
10.1002/bit.25984
[Indexed for MEDLINE]

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