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Mitochondrion. 2016 Sep;30:236-47. doi: 10.1016/j.mito.2016.08.012. Epub 2016 Aug 22.

Mitochondrial haplogroups and expression studies of commonly used human cell lines.

Author information

1
Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, D-12203 Berlin, Germany. Electronic address: Christine.Siegismund@ikdt.de.
2
University of Leipzig, Centre for Biotechnology and Biomedicine (BBZ), Molecular Cell Therapy, Deutscher Platz 5, D-04103 Leipzig, Germany.
3
Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, D-12203 Berlin, Germany; Department of Cardiology, Campus Virchow, Charité - University Hospital Berlin, Augustenburger Platz 1, D-13353, Germany.
4
Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, D-12203 Berlin, Germany.

Abstract

We developed a multiplex fragment length analysis (MFLA) for clearly assigning mitochondrial haplogroups mostly endemic in Europe for future cardiac diagnostics. As a technical proof, 23 commonly used human cell lines were haplotyped as reference standards. The functional analysis on mtDNA copies per cell revealed no correlation to haplogroups but a relatively high rate of mitochondria per cell and at the same time a very low expression of all mitochondrial and some nuclear encoded mitochondrial related genes. Established MFLA is an easy to handle method for analysing European mitochondrial haplogroups to perform epidemic studies and elucidate correlations to distinct diseases.

KEYWORDS:

Cell lines; Gene expression analysis; Mitochondria; Mitochondrial haplogroup; Multiplex fragment length analysis; mtDNA

PMID:
27562426
DOI:
10.1016/j.mito.2016.08.012
[Indexed for MEDLINE]

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