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Am J Physiol Gastrointest Liver Physiol. 2016 Oct 1;311(4):G624-G633. doi: 10.1152/ajpgi.00433.2015. Epub 2016 Aug 25.

Dual effects of a high-protein diet on DSS-treated mice during colitis resolution phase.

Author information

1
UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France; annaig.lan@agroparistech.fr.
2
UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France.
3
Laboratory of Applied Nutrition and Metabolism, School of Physical Education and Sports, University of São Paulo, Brazil.
4
UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France; Department of Gastroenterology, Hôpital Avicenne, Université Paris 13, Bobigny, France; and.
5
Service d'Anatomie et Cytologie Pathologiques, Hôpital Bichat-Claude Bernard, Paris, France.

Abstract

The impact of the dietary protein level on the process of colonic mucosal inflammation and subsequent recovery remains largely unknown. In this study, we fed DSS-treated mice with either a normoproteic (NP) or a high-protein (HP) isocaloric diet from the beginning of the 5-day dextran sulfate sodium (DSS) treatment to 14 days later. Measurements of colitis indicators (colon weight:length ratio, myeloperoxidase activity, cytokine expressions) showed a similar level of colonic inflammation in both DSS groups during the colitis induction phase. However, during the colitis resolution phase, inflammation intensity was higher in the DSS-HP group than in the DSS-NP group as evidenced by higher inflammatory score and body weight loss. This coincided with a higher mortality rate. In surviving animals, an increase in colonic crypt height associated with a higher number of colon epithelial cells per crypt, and TGF-β3 content was observed in the DSS-HP vs. DSS-NP group. Moreover, colonic expression patterns of tight junction proteins and E-cadherin were also different according to the diet. Altogether, our results indicate that the HP diet, when given during both the induction and resolution periods of DSS-induced colitis, showed deleterious effects during the post-induction phase. However, HP diet ingestion was also associated with morphological and biochemical differences compatible with higher colonic epithelium restoration in surviving animals, indicating an effect of the dietary protein level on colonic crypt repair after acute inflammation. These data highlight the potential impact of the dietary protein amount during the colitis course.

KEYWORDS:

DSS; colitis induction; colitis resolution; colonic crypt repair; high-protein diet

PMID:
27562061
DOI:
10.1152/ajpgi.00433.2015
[Indexed for MEDLINE]
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