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Angew Chem Int Ed Engl. 2016 Sep 19;55(39):12068-72. doi: 10.1002/anie.201606609. Epub 2016 Aug 24.

Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles.

Author information

1
Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore.
2
Institute of Chemical & Engineering Sciences, Agency for Science, Technology, and Research, 1 Pesek Road, Jurong Island, Singapore, 627833, Singapore.
3
Novartis Institute for Tropical Diseases, Singapore, Singapore.
4
School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
5
Department of Biochemistry & Molecular Biology, Department of Pharmacology & Toxicology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, USA.
6
Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore. cbkang@etc.a-star.edu.sg.

Abstract

Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.

KEYWORDS:

NMR spectroscopy; NS4B; dengue virus; membrane protein; membrane topology

PMID:
27554985
DOI:
10.1002/anie.201606609
[Indexed for MEDLINE]

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