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Elife. 2016 Aug 24;5. pii: e15593. doi: 10.7554/eLife.15593.

Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis.

Author information

1
CNRS UMR 7622, F-75005 Paris, France.
2
Sorbonne Universités, UPMC Univ Paris 06, Paris, France.
3
IBPS-Developmental Biology Laboratory, Paris, France.
4
Inserm U1156, F-75005, Paris, France.
5
Developmental Biology, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.

Abstract

The importance of mechanical activity in the regulation of muscle progenitors during chick development has not been investigated. We show that immobilization decreases NOTCH activity and mimics a NOTCH loss-of-function phenotype, a reduction in the number of muscle progenitors and increased differentiation. Ligand-induced NOTCH activation prevents the reduction of muscle progenitors and the increase of differentiation upon immobilization. Inhibition of NOTCH ligand activity in muscle fibers suffices to reduce the progenitor pool. Furthermore, immobilization reduces the activity of the transcriptional co-activator YAP and the expression of the NOTCH ligand JAG2 in muscle fibers. YAP forced-activity in muscle fibers prevents the decrease of JAG2 expression and the number of PAX7+ cells in immobilization conditions. Our results identify a novel mechanism acting downstream of muscle contraction, where YAP activates JAG2 expression in muscle fibers, which in turn regulates the pool of fetal muscle progenitors via NOTCH in a non-cell-autonomous manner.

KEYWORDS:

NOTCH; YAP; chick; chicken; developmental biology; limb; mechanics; myogenesis; stem cells

PMID:
27554485
PMCID:
PMC5030091
DOI:
10.7554/eLife.15593
[Indexed for MEDLINE]
Free PMC Article

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