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Chemistry. 2016 Oct 4;22(41):14475-9. doi: 10.1002/chem.201602795. Epub 2016 Aug 24.

Green Synthesis of Red-Emitting Carbon Nanodots as a Novel "Turn-on" Nanothermometer in Living Cells.

Author information

1
School of Chemical Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, P.R. China. wangcx@jiangnan.edu.cn.
2
School of Chemical & Material Engineering, Jiangnan University, Wuxi, 214122, P. R. China. wangcx@jiangnan.edu.cn.
3
School of Chemical & Material Engineering, Jiangnan University, Wuxi, 214122, P. R. China.
4
School of Chemical Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, P.R. China. chi.zhang@jiangnan.edu.cn, chizhang@tongji.edu.cn.
5
School of Chemical & Material Engineering, Jiangnan University, Wuxi, 214122, P. R. China. chi.zhang@jiangnan.edu.cn, chizhang@tongji.edu.cn.

Abstract

Temperature measurements in biology and medical diagnostics, along with sensitive temperature probing of living cells, is of great importance; however, it still faces significant challenges. Herein, a novel "turn-on" carbon-dot-based fluorescent nanothermometry device for spatially resolved temperature measurements in living cells is presented. The carbon nanodots (CNDs) are prepared by a green microwave-assisted method and exhibit red fluorescence (λem =615 nm) with high quantum yields (15 %). Then, an on-off fluorescent probe is prepared for detecting glutathione (GSH) based on aggregation-induced fluorescence quenching. Interestingly, the quenched fluorescence could be recovered by increasing temperature and the CNDs-GSH mixture could behave as an off-on fluorescent probe for temperature. Thus, red-emitting CNDs can be utilized for "turn-on" fluorescent nanothermometry through the fluorescence quenching and recovery processes, respectively. We employ MC3T3-E1 cells as an example model to demonstrate the red-emitting CNDs can function as "non-contact" tools for the accurate measurement of temperature and its gradient inside a living cell.

KEYWORDS:

carbon nanodots; fluorescence; green chemistry; nanoparticles; nanotechnology

PMID:
27553910
DOI:
10.1002/chem.201602795
[Indexed for MEDLINE]

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