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Adv Exp Med Biol. 2016;922:151-160. doi: 10.1007/978-3-319-35072-1_11.

Serial Femtosecond Crystallography of Membrane Proteins.

Author information

1
School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287, USA.
2
Center for Applied Structural Discovery at the Biodesign Institute, Arizona State University, Tempe, AZ, 85287-1604, USA.
3
Department of Physics, Arizona State University, Tempe, AZ, 85287, USA.
4
Bridge Institute, University of Southern California, Los Angeles, CA, 90089, USA.
5
Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, USA.
6
School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287, USA. w.liu@asu.edu.
7
Center for Applied Structural Discovery at the Biodesign Institute, Arizona State University, Tempe, AZ, 85287-1604, USA. w.liu@asu.edu.

Abstract

Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals. Recent developments of serial femtosecond crystallography at X-ray free electron lasers combined with the use of membrane-mimetic gel-like matrix of lipidic cubic phase (LCP-SFX) for crystal growth and delivery hold significant promise to accelerate structural studies of membrane proteins. This chapter describes the development and current status of the LCP-SFX technology and elaborates its future role in structural biology of membrane proteins.

KEYWORDS:

G protein-coupled receptors; LCP injector; LCP-SFX; Lipidic cubic phase; Membrane proteins; Serial femtosecond crystallography; X-ray free electron laser

PMID:
27553241
PMCID:
PMC5110035
DOI:
10.1007/978-3-319-35072-1_11
[Indexed for MEDLINE]
Free PMC Article

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