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Avian Pathol. 2017 Feb;46(1):90-94. doi: 10.1080/03079457.2016.1222808. Epub 2016 Dec 2.

Evaluation of a selected lactic acid bacteria-based probiotic on Salmonella enterica serovar Enteritidis colonization and intestinal permeability in broiler chickens.

Author information

1
a Facultad de Medicina Veterinaria y Zootecnia , Universidad de Colima , Colima , México.
2
b Centro Universitario de Investigación y Desarrollo Agrícola , Universidad de Colima , Colima , México.
3
c Departamento de Producción Agrícola y Animal , Universidad Autónoma Metropolitana , México City , México.
4
d Department of Poultry Science, Center of Excellence for Poultry Science , University of Arkansas , Fayetteville , AR , USA.
5
e Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia , Universidad Nacional Autónoma de México , México City , México.

Abstract

Two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (FloraMax-B11®) against Salmonella enterica serovar Enteritidis intestinal colonization and intestinal permeability in broiler chickens. Experiment 1 consisted of two independent trials. In each trial, day-old broiler chicks were assigned to one of two groups: control + S. Enteritidis or probiotic + S. Enteritidis. At 72 h post-S. Enteritidis challenge, haematology and caecal content were evaluated for S. Enteritidis colonization. In Experiment 2, day-old broiler chicks were assigned to one of four groups: negative control; probiotic; control + S. Enteritidis; or probiotic + S. Enteritidis. At 72 h post-S. Enteritidis challenge, chickens in all groups were given an oral gavage dose of fluorescein isothiocyanate dextran (FITC-d). In both trials of Experiment 1, a significant reduction (P < 0.05) in colony-forming units/gram of S. Enteritidis in caecal content and a reduction in the incidence of S. Enteritidis enriched caecal samples were observed in probiotic + S. Enteritidis chickens. In addition, significant heterophilia and lymphopaenia were observed in control + S. Enteritidis chickens. In Experiment 2, a decrease in numbers of S. Enteritidis in caeca were observed in probiotic + S. Enteritidis chickens when compared to control + S. Enteritidis. Also, an increase in serum FITC-d concentration was detected in control + S. Enteritidis. These results suggest that early infection with S. Enteritidis can increase intestinal permeability, but the adverse effects can be prevented by the administration of the probiotic tested.

KEYWORDS:

Broiler; Salmonella Enteritidis; intestinal permeability; probiotic

PMID:
27545145
DOI:
10.1080/03079457.2016.1222808
[Indexed for MEDLINE]

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