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Biochim Biophys Acta. 2016 Nov;1857(11):1759-1765. doi: 10.1016/j.bbabio.2016.08.006. Epub 2016 Aug 18.

Pigment structure in the FCP-like light-harvesting complex from Chromera velia.

Author information

1
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, F-91198 Gif-sur-Yvette cedex, France. Electronic address: mjllansola@gmail.com.
2
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, F-91198 Gif-sur-Yvette cedex, France.
3
Institute of Plant Molecular Biology, Biology Centre CAS, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic; Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.

Abstract

Resonance Raman spectroscopy was used to evaluate pigment structure in the FCP-like light-harvesting complex of Chromera velia (Chromera light-harvesting complex or CLH). This antenna protein contains chlorophyll a, violaxanthin and a new isofucoxanthin-like carotenoid (called Ifx-l). We show that Ifx-l is present in two non-equivalent binding pockets with different conformations, having their (0,0) absorption maxima at 515 and 548nm respectively. In this complex, only one violaxanthin population absorbing at 486nm is observed. All the CLH-bound carotenoid molecules are in all-trans configuration, and among the two Ifx-l carotenoid molecules, the red one is twisted, as is the red-absorbing lutein in LHCII trimers. Analysis of the carbonyl stretching region for Chl a excitations indicates CLH binds up to seven Chl a molecules in five non-equivalent binding sites, in reasonable agreement with sequence analyses which have identified eight potential coordinating residues. The binding modes and conformations of CLH-bound pigments are discussed with respect to the known structures of LHCII and FCP.

KEYWORDS:

CLH; Carotenoids; Chlorophylls; Light harvesting complexes; Photosynthesis; Resonance Raman

PMID:
27544823
DOI:
10.1016/j.bbabio.2016.08.006
[Indexed for MEDLINE]
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