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J Biosci Bioeng. 2017 Jan;123(1):134-138. doi: 10.1016/j.jbiosc.2016.07.008. Epub 2016 Aug 16.

Development of a liquid chromatography-tandem mass spectrometry method for quantitative analysis of trace d-amino acids.

Author information

1
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
2
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: fukusaki@bio.eng.osaka-u.ac.jp.

Abstract

d-Amino acids have recently attracted much attention in various research fields including medical, clinical and food industry due to their important biological functions that differ from l-amino acid. Most chiral amino acid separation techniques require complicated derivatization procedures in order to achieve the desirable chromatographic behavior and detectability. Thus, the aim of this research is to develop a highly sensitive analytical method for the enantioseparation of chiral amino acids without any derivatization process using liquid chromatography-tandem mass spectrometry (LC-MS/MS). By optimizing MS/MS parameters, we established a quantification method that allowed the simultaneous analysis of 18 d-amino acids with high sensitivity and reproducibility. Additionally, we applied the method to food sample (vinegar) for the validation, and successfully quantified trace levels of d-amino acids in samples. These results demonstrated the applicability and feasibility of the LC-MS/MS method as a novel, effective tool for d-amino acid measurement in various biological samples.

KEYWORDS:

Enantioseparation; Liquid chromatography-tandem mass spectrometry; Quantitative analysis; Underivatization; d-Amino acids

PMID:
27542694
DOI:
10.1016/j.jbiosc.2016.07.008
[Indexed for MEDLINE]

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